CAT number: 5051 - Basic Kit

CAT number: B5051 - Bacterial Strain Kit

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The Muta-ChromoPlate kit™ is a 96 well microplate version of the salmonella typhimurium Ames Test, used for detection of mutagenic activity (Follows the OECD 471 Guidelines for Testing Of Chemicals - Bacterial Reverse Mutation Test).

Developed to test mutagenic materials in water soluble extracts of , sediment, air, chemicals, food components, cosmetics, waste waters, potable waters and  any other material that can be solubilized or placed into micro suspension in water such that the material being tested can be taken up by the test strain.

• The Muta-ChromoPlate provides a clear colour endpoint.

  Reagents, cultures and other consumable components are supplied ready-to-use in a non-specialized laboratory. The Muta-ChromoPlate™ kit is based on the most generally used bacterial reverse mutation test, known as the Ames Test (Ames et al., 1975 Mutation Research 31:347)

  The test employs a mutant strain, or several strains, of Salmonella typhimurium, carrying mutation(s) in the operon coding for histidine biosynthesis. When these bacteria are exposed to mutagenic agents, under certain conditions, reverse mutation from amino acid (histidine) auxotrophy to prototrophy occurs.

  Traditionally, reverse-mutation assays have been performed using agar plates. An alternate assay performed entirely in liquid culture is the 'Fluctuation Test' based on multiple yes/no colour endpoints (Hubbard, S.A et al., 1994, pp. 141-160, in Kilbey et al. (Eds.), Handbook of Mutagenicity Testing (2nd Ed,.) Elsevier Sciences, NY. This test principle is being applied in the Muta-ChromoPlate test kit.

  The Muta-ChromoPlate™ Kit is generally more sensitive (up to 10 times) than the pour-plate assay, because it allows testing of higher concentrations of sample (up to 75% v/v). The assay procedure is simple and requires minimal training. Consumable components are provided with ready-to-use and step-by-step instructions. "Instructions for Use" are provided with the basic kit. The only equipment required are a 37 degree Celsius incubator and a single and a multi-channel micropipettor.

• For a visual understanding on how the Muta-ChromoPlate™ kit is run.

• More details

• EBPI has developed an excel application for the Muta-ChromoPlate™ kit which allows for quick and easy results of your analysis. For a PDF version of the instructions, please contact EBPI for a copy of the excel application.
• Applications:

  Testing of pharmaceuticals for mutagenic activity.

  Testing of industrial effluents for presence of possible mutagenic compounds.

  Screening of municipal discharges for possible routine presence or spills of mutagenic compounds.

  Screening of surface and ground water for mutagenic residues.

  Screening of potable water supplies for the presence of chemicals with mutagenic potential.

  Screening of water soluble air pollutants for mutagenic agents.Evaluation of pure or complexed raw mixtures for potential mutagenicity.A convenient and easy to use teaching look for university and college laboratories.The Muta-ChromoPlate kit (Ames Test Kit) is designed to be user friendly by removing potential contamination concerns.

Reverse Mutations in Various Bacterial Strains

There are several strains of S. typhimurium and E.coli which are used for the Ames Test. Each strain incorperates different mutations in their histidine biosynthesis gene, making them sensitive to different types of mutations. This allows the Ames Test to be more sensitive to different types of chemicals.

More details

Strains Currently Available to be shipped are: TA97a, TA98, TA100, TA102 and the TA1535; as well as the WP2 urvA and the WP2 urvA (pKM101).