Acute Toxicity Tests
EBPI has developed two acute bacterial based toxicity tests.
1. Toxi-ChromoTest kit
2. Toxi-ChromoPad kit
In 2006, the Toxi-ChromoTest was verified by the ETV program under the U.S. Environmental Protection AgencyMore details .
- Ames Test Kit
- SOS-ChromoTest Kit
- Toxicity Testing
- E.Coli Testing
- MicroBioTests
- Algae Toxicity Tests
- Artemia Toxicity Tests
- Daphnia Toxicity Tests
- Ostracod Toxicity Tests
- Phyto-Toxicity Tests
- Protozoan Toxicity Tests
- Rotifer Toxicity Tests
- Thamnocephalus Toxicity
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The Toxi-ChromoTest kit™ is designed for Rapid Detection of Acute or Chronic Toxicity.
CAT Number: 5032
The procedure exposes the bacteria to the toxicants in the sample for a short (90-minute) incubation period. After the incubation period, a chromogenic substrate is added. If the sample is toxic, no colour will develop and if the sample is non-toxic, a distinctive blue colour quickly develops. The kit provides a clear, completely objective measurement of the toxicity of the sample by a simple visual qualitative evaluation of the colour obtained, or quantitatively by spectrophotometry using a micro-plate reader.
The Toxi-ChromoTest™ kit contains three sets of components and, therefore, allows four separate testing procedures to be completed at the same or different times.
Each Toxi-ChromoTest™ kit can be used for a total of 192 tests. These tests must include standards and controls (using at least 32 units.) Thus, a single kit can screen 160 samples for yes/no response without replicates.
It is necessary to allow sample dilutions and replicates, thus testing of no more than 20 samples with each kit for reliable and quantitative bioassays is recommended.
Applications for the Toxi-ChromoTest™ include screening of effluents for changes in toxicity levels, monitoring of municipal discharges for occasional spills, inclusion in a "battery of tests" for monitoring aqueous environmental samples, detection of potential toxicity in raw materials, and use as a convenient research and teaching tool.
Used within Universities across North America, the Toxi-ChromoTest™ kit can be tailored to meet the demands of any university student. Used in support of a graduate thesis, or as a learning tool for laboratory use at the undergraduate level.
A visual guide to the Toxi-ChromoTest™ kits
Productivity
The Toxi -ChromoTest™ offers the following advantages over traditional toxicity testing methods:
Testing of pharmaceuticals for toxic activity.
Testing of industrial effluents for presence of possible toxic compounds.
Screening of municipal discharges for possible routine presence or spills of toxic compounds.
Screening of surface and ground water for toxic residues.
Screening of potable water supplies for the presence of chemicals with toxic potential.
Screening of water soluble air pollutants for toxic agents.
Evaluation of pure or complexed raw mixtures for potential toxicity.
A convenient and easy to use teaching look for university and college laboratories.
The Toxi-ChromoTest™ kit is designed to be user friendly, reliable, sensitive, rapid, low cost and contains all plastics needed to complete the assay.
Examples of toxicants tested by the Toxi-ChromoTest™ kit (Toxic response of bacteria is depicted in graphic form, relating to the percentage of toxicity to concentration of various substances).
The advantages of bacterial tests:
In comparison with higher organisms, bacteria have the same biopathways, possess similar semi permeable membranes, and are therefore vulnerable to toxic substances which either affect the membrane or biosynthetic pathways. In addition, bacteria have the advantage of rapid growth and enable the use of very large populations. Their cultivation is economical and one can use very small sample volumes and allow for easy standardization of the technique.
EBPI has developed an excel application for the Toxi-ChromoTest™ kit which allows for quick and easy results of your analysis. For a PDF version of the instructions, please contact EBPI for a copy of the excel application.
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The Toxi-ChromoPad™ test is designed for Rapid Detection of Acute Toxicity during Contact. The Toxi-ChromoPad™ bioassay uses a novel mutant of the bacterium E.coli as the test organism. This bacterium was selected specifically for sensitivity to a wide range of toxic substances.
CAT Number: 5032
The assay allows the bacteria to grow in direct contact with the toxicants in the sample, eliminating expensive extraction procedures. The endpoint of the assay is an easy to interpret colour reaction. If the sample is toxic, no colour will develop; if the sample is non-toxic, a distinctive blue colour develops around the sample.
The Toxi-ChromoPad™ is a bioassay screening tool used to determine acute toxicity in soils, sediments, sludge or other solid waste materials directly without extraction.
Each kit contains all the reagents and disposable materials necessary to test at least four samples, including dilutions and controls.
Used within Universities across North America, the Toxi-ChromoPad™ kit can be tailored to meet the demands of any university student. Used in support of a graduate thesis, or as a learning tool for laboratory use at the undergraduate level.
The Toxi-ChromoPad™ offers the following advantages over traditional methods: Testing of industrial solid effluents for presence of possible toxic compounds. Screening of municipal soils for possible routine presence or spills of toxic compounds. Evaluation of pure or complexed raw mixtures for potential toxicity in sediments. A convenient and easy to use teaching look for university and college laboratories. The Toxi-ChromoPad™ kit is designed to be user friendly, reliable, sensitive, rapid,and low cost. Contains all plastics needed to complete the assay . |
