Mutagenicity Tests

 

Muta-ChromoPlate™ Basic Kit

 muta ChromoPlate Ames logo2muta ChromoPlate BasicKit Ames Testing

5051 Muta-ChromoPlate Basic Kit (Contains 1 bacterial, reagents, controls and plastics) 

B5051 Muta-ChromoPlate Bacterial Strain Kit (Contains 2 bacterial reagents, controls and plastics)

5051S9 Activation Enzymes (designed for the Ames Test)

A microplate version of the traditional "pour plate" Ames Test.  The Muta-ChromoPlateTM Kit is sensitive and user friendly. It is commonly used to detect mutagenic potential of individual chemicals or mixtures of chemicals in water, wastewater, sediment, air aqueous extracts, new chemicals, foods, cosmetics, pharmaceuticals and any other material that can be put into aqueous solution or micro-suspension, such that it can be taken up by the test strain.

 

Ames 384-ISO™ Well Test

ames 384 ISO Well Format logoames 384 ISO full kit large

6041 Ames-384 ISO Basic Kit (Contains 1 bacterial, reagents, controls and plastics)

6041-2BS Ames-384 ISO Bacterial Strain Kit (Contains 2 bacterial reagents, controls and plastics)

The Ames ISO Basic Kit is a fluctuation assay verison of the well-known “Ames Test” which uses a 48-well sample exposure format on a 384-well microplate to reduce reagent and plastic used.  The Ames ISO procedure was developed to test mutagenic materials in a variety of sources like environmental air and water samples, foods, personal care products, and industrial effluents by the fluctuation assay which is a variation of the traditional “Ames Test”.

The Ames ISO method provides researchers with a reliable test method which is facile to set up and run, amenable to samples in aqueous solution or suspension and produces an easily-detectable colorimetric endpoint after 2-3 days of incubation at 37 °C.

 

Ames Modified ISO™ Test

ames MOD ISO logoames MOD ISO Test Kit large

5041 Ames Modified ISO Basic Kit (Contains 1 bacterial, reagents, controls and plastics)

5041-2BS Ames Modified ISO Bacterial Strain Kit (Contains 2 bacterial reagents, controls and plastics)

The Ames ISO Basic Kit is a fluctuation assay verison of the well-known “Ames Test” which uses a 48-well sample exposure format on a 384-well microplate to reduce reagent and plastic used. This Ames test method is a modification of the Ames ISO fluctuation assay. Bacterial strains are pre-exposed to differing concentrations of the mutagenic compounds with a growth media in suspension for 100 minutes. Each dilution is run in triplicate.

The samples are then transferred, mixed with a reversion solution and plated onto a 96-well plate while using a 48-well evaluation format. The samples are incubated for 2-3 days and the number of revertant colonies (individual wells) are counted by colorimetric analysis.

 

Which Ames Test is best for my research

 
Muta-ChromoPlate Ames Test
  • Ideal for direct assessment of environmental samples
  • Educational uses for classroom projects (easy to preform)
  • 96 well format on 96 well plates. More endpoints/ sample can improve significance
  • Bacterial exposure occurs throughout the assay
  • Extremely user friendly with minimal steps required to obtain results
  • Excellent to test volatile materials
  • No preconcentration steps required, large sample volumes used
  • More sensitive results compared to plate incorporation assays
 
ISO and Modified ISO Ames Tests
  • Ideal for pharmaceutical and novel molecule discoveries
  • 48-well format in 96 or 384-well plates reduces plates required for result development
  • HIGH THROUGHPUT METHOD! Many samples or dilution levels can be tested rapidly
  • Smaller sample sizes required
  • Bacterial concentration is checked by optical density (ensures log growth phase) 
  • Reduced plastics and reagents necessary, less disposal
  • Quantification is simple and test scoring is quick and easy
  • Assay is more sensitive than the plate-incorporation assay
  • Utilizes pre-exposure period to enhance bacterial uptake
  • More consistent exposure concentration during auxotrophic growth phases

Ames Test Kits

 

EBPI offers a wide variety of Ames test methods for research and development, environmental sampling, and drug discovery projects.

 ames Test Background Plate

Muta-ChromoPlate Kit

ames 384 ISO Test plate 

Ames ISO Test 384 Well Format

ames MOD ISO Plate 

Modified Ames ISO Test