Algae Toxicity Tests

Fresh Water Algae Toxicity Test

freshWater Marine Toxicity Tests

Algaltoxkit F Freshwater Algae Toxicity Test

ALGALTOXKIT F is a Cost-effective, culture and maintenance free bioassays with micro-algae Selenastrum capricornutum (renamed Raphidocelis subcapitata/Pseudokirchneriella subcapitata).  72 hour microbiotest for Toxicity screening of pure compounds, effluents, sediments, wastewaters, and surface and ground waters.

Each ALGALTOXKIT F contains all the materials necessary to perform two growth inhibition tests with the freshwater microalgae Selenastrum capricornutum. Easy to follow instructions and detailed illustrations are provided in the kits for the conduct of range-finding and definitive tests. The test organisms are included in the kits in algal beads from which the micro-algae can easily be set free on demand, in less than 30 min, to supply the live biota for the conduct of the assays.

Product Number TK41 ALGALTOXKIT F

REQUEST A QUOTE

Resources:

Please review EBPI's step-by-step presentation on the Fresh Water Algaltoxkit F Procedure:

ALGALTOXKIT F Presentation

algaltoxkitF FreshWaterAlgae Presentation

Click here to download the ALGALTOXKIT F Power Point.

MicroBioTests Comparison Data

comparison Data MicroBiotests

Click here to download comparison data sheet.

TK41 ALGALTOXKIT F Short Protocol

freshWater Marine ToxicityTests

Click here to download instructions.

Reproducibility

  • Algal beads of high quality produced in strictly controlled conditions preclude variability associated with recruitment/ maintenance of livestock’s of micro-algae in exponential growth phase, in conventional bioassays.
  • Highly uniform quality of the test medium is achieved by simple dilution of Concentra-ted solutions of selected chemicals, with deionizer water.
  • Standardized “long cell” test containers (10 cm path length) constructed of biologically inert materials ensure uniform exposure conditions.
  • A Quality Control Test with a reference chemical is described in detail, for accuracy and reproducibility check.

Cost-Effective

  • The ALGALTOXKIT makes use of unique disposable “long cell” exposure chambers (10 cm path length), which also serve as measuring curettes for direct determination of algal growth by optical density measurements.
  • Labour time for testing is significantly reduced by the direct measurement of algal growth in the long cells in a (suited) spectrophotometer.
  • Shelf-life of the algal beads is guaranteed for several months when stored properly, reducing test scheduling constraints

Contents

  • Tubes with algal beads, concentrated algal growth and toxicant dilution medium, long cells and holding trays.
  • Detailed Standard Operational Procedure brochure, abbreviated Bench Protocol, OD/N regression sheet, data scoring sheets and graphical EC50 calculation sheets.
  • Specification sheet with batch number of the algal beads and the media.

User-Friendly

  • Bioassays can be started within 30 min. from the “de-immobilization” of the microalgae from the beads
  • Simple handlings and scorings
  • Daily scoring of the optical density of the micro-algae in the long cells takes less than 15 min.

Sensitivity

  • Comparable to the sensitivity of the algal growth inhibition test with micro-algae from laboratory cultures.

Validation

  • The ALGALTOXKIT is used in many laboratories worldwide for routine screening of chemicals and environmental samples·
  • Substantial number of publications reporting data for various uses.
  • The ALGALTOXKIT Microbiotests strictly adheres to the protocols for regulatory testing with micro-algae, prescribed by international organizations such as e.g. the OECD and the ISO.
  • The Algal beads that are 2mm in size contain more than 1 million algal cells per bead.  The micro-algae are designed to be set free in demand so that the user can focus on the testing and not on the production of the algae.

freshWater Marine ToxicityTests

 tk41 algal FreshWater Toxicity Test 

Once the Algal beads are set free, the algal cells are ready for testing.   Each Algaltoxkit contains enough materials to perform two 72hr growth inhibition tests.

Optional Components for the ALGALTOXKIT F

  • TK41     Algaltoxkit F Selenastrum with Selenastrum capricornumtum (2 tests per Toxkit)

Test Biota

  • TB41     5 Tubes with Selenastrum capricornutum beads + de-immobilization medium

Test containers

  • TC41     50 long cells with lids
  • TC42     5 holding trays for long cells
  • TC43     5 holding trays with long cells and lids

Test Media

  • TM31     5 sets of concentrated media for algal tests (ISO medium for culturing)
  • TM35     5 vials of nutrient stock A for freshwater algal tests

Equipment Needed

  • Spectrophotometer
  • Luxmeter
  • Incubator